Composite

Part:BBa_K2240000:Design

Designed by: iGEM2017_HKUST   Group: iGEM17_Hong_Kong_HKUST   (2017-10-18)


AHL sensor with positive feedback loop, GFP output and antisense RNA type 1 inhibition


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1751
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal BsaI.rc site found at 2455
    Illegal BsaI.rc site found at 2674


Design Notes

NIL


Source

Most of the part was obtained from the iGEM Part Registry, except for the sequences of the antisense RNA type 1 together with the Hfq binding region (BBa_K2240002), and the antisense RNA binding region type 1 (BBa_K2240001).


References

Hoynes-O’Connor, A., & Moon, T. S. (2016). Development of design rules for reliable antisense RNA behavior in E. coli. ACS synthetic biology, 5(12), 1441-1454.

Wagner, E. G. H. (2009). Kill the messenger: bacterial antisense RNA promotes mRNA decay. Nature structural & molecular biology, 16(8), 804-806.